Hotspot

trnS-GCU-trnG-UCC

LSC · 52635-53647 · 1013 bp

Back to Pseudalcantarea

Pi 0.00412

MarkerSeek score 65.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 4
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	55.0	50.0	58.0	58.3	1137-1180 bp	1.0	80.4
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	1082-1125 bp	1.0	80.4
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	55.0	50.0	58.0	57.5	1138-1181 bp	1.0	79.1
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	1083-1126 bp	1.0	79.1
trnS-GCU-trnG-UCC_p5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	55.0	50.0	58.0	57.4	1136-1179 bp	1.0	78.7