Hotspot

rps12-rps7

IRb · 30872-30929 · 58 bp

Back to Pseudalcantarea

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRb
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	CTGTGGAAAGCCGTATTCGA	TTCTTCTGCAGTACCTCGAC	50.0	50.0	58.0	57.0	190 bp	1.0	72.3
rps12-rps7_p2	TCTGTGGAAAGCCGTATTCG	TTCTTCTGCAGTACCTCGAC	50.0	50.0	58.0	57.0	191 bp	1.0	72.3
rps12-rps7_p3	CTGTGGAAAGCCGTATTCGA	TCTTCTGCAGTACCTCGACG	50.0	55.0	58.0	59.2	189 bp	1.0	70.8
rps12-rps7_p4	TCTGTGGAAAGCCGTATTCG	TCTTCTGCAGTACCTCGACG	50.0	55.0	58.0	59.2	190 bp	1.0	70.8
rps12-rps7_p5	CTGTGGAAAGCCGTATTCGA	TTTCTTCTGCAGTACCTCGAC	50.0	47.6	58.0	57.7	191 bp	1.0	70.0