Hotspot

ndhG-ndhI

SSC · 8820-9206 · 387 bp

Back to Pseudalcantarea

Pi 0.00775

MarkerSeek score 44.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 387
variable_sites: 3
pi: 0.00775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCTGGTTTGACCTTGCAGTC	GGGGTCGAATCCACTTTGAA	50.0	50.0	58.0	57.8	1164 bp	1.0	78.7
ndhG-ndhI_p2	CCCCAGACCCCCTAATACAA	GGGGTCGAATCCACTTTGAA	55.0	50.0	58.1	57.8	845 bp	1.0	78.3
ndhG-ndhI_p3	TGTGCGGCAGCTACAAAATA	GGGGTCGAATCCACTTTGAA	45.0	50.0	58.2	57.8	955 bp	1.0	77.6
ndhG-ndhI_p4	TCTGGTTTGACCTTGCAGTC	ATCACATCAGAGCGTTTCCG	50.0	50.0	58.0	58.4	1183 bp	1.0	77.4
ndhG-ndhI_p5	TCAAAGTGACCCCATCTCCA	GGGGTCGAATCCACTTTGAA	50.0	50.0	58.3	57.8	1074 bp	1.0	76.9