Hotspot

ndhF-rpl32

SSC · 2215-3212 · 998 bp

Back to Pseudalcantarea

Pi 0.00302

MarkerSeek score 60.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 998
variable_sites: 3
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	40.0	50.0	55.3	58.3	1218 bp	1.0	56.6
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAGTAGA	GGTGGAAACCGATTTCGCTA	40.9	50.0	57.2	58.3	1217 bp	1.0	55.9
ndhF-rpl32_p3	CTGGAAGTGGAAGAAAAGGT	GGTGGAAACCGATTTCGCTA	45.0	50.0	55.2	58.3	1170 bp	1.0	55.6
ndhF-rpl32_p4	TTTGTTGCTGTCGGAATAAGT	GGTGGAAACCGATTTCGCTA	38.1	50.0	56.1	58.3	1219 bp	1.0	55.0
ndhF-rpl32_p5	TGCTGTCGGAATAAGTAGAAGTC	GGTGGAAACCGATTTCGCTA	43.5	50.0	58.0	58.3	1214 bp	1.0	54.2