Hotspot

trnH-GUG

LSC · 81736-81810 · 75 bp

Back to Psathyrostachys

Pi 0.0267
MarkerSeek score 67.80
Primer available yes
Label trnH-GUG

Feature Metadata

label_name
trnH-GUG
feature_type
tRNA
region
LSC
length_bp
75
variable_sites
2
pi
0.0267
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG_p1 TCGAAGTACGGAGGTCAGAA TCTTCGTCGCCGTAAGTAAA 50.0 45.0 57.8 57.0 228 bp 1.0 71.3
trnH-GUG_p2 TCGAAGTACGGAGGTCAGAA TTCTTCGTCGCCGTAAGTAA 50.0 45.0 57.8 57.0 229 bp 1.0 71.3
trnH-GUG_p3 TCGAAGTACGGAGGTCAGAA CGTCGCCGTAAGTAAATACG 50.0 50.0 57.8 56.9 224 bp 1.0 70.0
trnH-GUG_p4 TCGAAGTACGGAGGTCAGAA GTTTTATTCTTCGTCGCCGT 50.0 45.0 57.8 56.9 235 bp 1.0 70.0
trnH-GUG_p5 TCGAAGTACGGAGGTCAGAA ATTCTTCGTCGCCGTAAGTA 50.0 45.0 57.8 56.8 230 bp 1.0 69.5