Hotspot

rpl2

IRb · 81871-83268 · 1398 bp

Back to Psathyrostachys

Pi 0.0151

MarkerSeek score 57.60

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1398
variable_sites: 21
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TCGCCCATCGCATTATTGC	CAAAACACCTATCCCGAGCA	52.6	50.0	59.1	57.9	1533 bp	1.0	64.1
rpl2_p2	TCGCCCATCGCATTATTGC	AAAACACCTATCCCGAGCAC	52.6	50.0	59.1	57.9	1532 bp	1.0	64.1
rpl2_p3	TCGCCCATCGCATTATTGC	ACAAAACACCTATCCCGAGC	52.6	50.0	59.1	57.9	1534 bp	1.0	64.1
rpl2_p4	TCGCCCATCGCATTATTGC	CCGTAGACAGGCAAGTGAAA	52.6	50.0	59.1	57.8	1501 bp	1.0	63.7
rpl2_p5	TCGCCCATCGCATTATTGC	GAACCGTAGACAGGCAAGTG	52.6	55.0	59.1	58.6	1504 bp	1.0	60.4