Hotspot

ndhH

IRb · 102653-102904 · 252 bp

Back to Psathyrostachys

Pi 0.0932

MarkerSeek score 75.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: IRb
length_bp: 252
variable_sites: 22
pi: 0.0932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.0	58.0	639-646 bp	1.0	85.3
ndhH_p2	ATCCCGTGAAATTCTCGAGC	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.1	58.0	1140-1147 bp	1.0	82.5
ndhH_p3	CTTTTGGGAAAACGTCGACG	AACACTTTTGCCTATCCGCA	50.0	45.0	58.0	58.1	1147-1154 bp	1.0	82.4
ndhH_p4	CTTTTGGGAAAACGTCGACG	ACACTTTTGCCTATCCGCAT	50.0	45.0	58.0	57.9	1146-1153 bp	1.0	82.1
ndhH_p5	ATCCCGTGAAATTCTCGAGC	AACACTTTTGCCTATCCGCA	50.0	45.0	58.1	58.1	1648-1655 bp	1.0	81.1