Hotspot

ndhF

SSC · 102920-105139 · 2220 bp

Back to Psathyrostachys

Pi 0.0018

MarkerSeek score 43.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	CGGAAGCCTATCCCCCATAT	45.0	55.0	58.0	58.4	2938-2944 bp	1.0	77.8
ndhF_p2	AATCGCGGAAAACCGAACTA	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.9	58.4	2936-2942 bp	1.0	77.5
ndhF_p3	TCGCGGAAAACCGAACTATT	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.9	58.4	2934-2940 bp	1.0	77.5
ndhF_p4	AAAATCGCGGAAAACCGAAC	ATGGTACGGAAGCCTATCCC	45.0	55.0	58.0	58.7	2944-2950 bp	1.0	76.0
ndhF_p5	ATCGCGGAAAACCGAACTAT	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.7	58.4	2935-2941 bp	1.0	75.7