Hotspot

ndhH-ndhF

SSC · 102905-102919 · 15 bp

Back to Psathyrostachys

Pi 0.533

MarkerSeek score 72.50

Primer available yes

Label ndhH-ndhF

Feature Metadata

label_name: ndhH-ndhF
feature_type: igs
region: SSC
length_bp: 15
variable_sites: 8
pi: 0.533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhF_p1	AAAATCGCGGAAAACCGAAC	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.0	58.0	239-246 bp	1.0	91.3
ndhH-ndhF_p2	ACCCATCAATGCATGGTGTT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	340-347 bp	1.0	89.9
ndhH-ndhF_p3	AACACCCATCAATGCATGGT	ATGGAGTGGGTCTTGCTAGT	45.0	50.0	58.1	58.0	343-350 bp	1.0	89.8
ndhH-ndhF_p4	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	50.0	50.0	58.0	58.0	639-646 bp	1.0	85.3
ndhH-ndhF_p5	CTTTTGGGAAAACGTCGACG	AACACTTTTGCCTATCCGCA	50.0	45.0	58.0	58.1	1147-1154 bp	1.0	82.4