Hotspot

petN-trnC-GCA

LSC · 18311-19031 · 721 bp

Back to Psathyrostachys

Pi 0.0154
MarkerSeek score 62.20
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
721
variable_sites
11
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTCCTTCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 829-1042 bp 1.0 72.1
petN-trnC-GCA_p2 GTCCACTCCTTCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 828-1041 bp 1.0 72.1
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 770-983 bp 1.0 71.9
petN-trnC-GCA_p4 AGTCCACTCCTTCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 831-1044 bp 1.0 71.6
petN-trnC-GCA_p5 GTCCACTCCTTCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 830-1043 bp 1.0 71.6