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psbM-petN

LSC · 17474-18220 · 747 bp

Back to Psathyrostachys

Pi 0.00679

MarkerSeek score 64.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 5
pi: 0.00679
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	42.9	55.0	57.6	58.1	841-851 bp	1.0	69.3
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	42.9	55.0	57.6	58.1	840-850 bp	1.0	69.3
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	899-909 bp	1.0	68.9
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	42.9	55.0	57.6	57.8	839-849 bp	1.0	68.8
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	42.9	60.0	57.6	58.2	836-846 bp	1.0	68.5