Hotspot

trnD-GUC-psbM

LSC · 16565-17368 · 804 bp

Back to Psathyrostachys

Pi 0.00674

MarkerSeek score 65.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 5
pi: 0.00674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	855-915 bp	1.0	62.8
trnD-GUC-psbM_p2	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	856-916 bp	1.0	58.6
trnD-GUC-psbM_p3	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.9	55.8	869-929 bp	1.0	57.4
trnD-GUC-psbM_p4	TCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.8	55.8	854-914 bp	1.0	56.8
trnD-GUC-psbM_p5	GCGGGGATTGTAGTTCAATTG	AGAATGAACAGTGCAGTAGC	47.6	45.0	57.6	55.8	868-928 bp	1.0	55.2