Hotspot

trnG-UCC-trnT-GGU

LSC · 14190-15385 · 1196 bp

Back to Psathyrostachys

Pi 0.0151
MarkerSeek score 58.50
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1196
variable_sites
18
pi
0.0151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 38.1 47.6 55.5 57.5 1274-1275 bp 1.0 41.5
trnG-UCC-trnT-GGU_p2 AGAACGAATCACACTTTTACCAC TGACTTACGTCTTAGCACGAG 39.1 47.6 57.3 57.5 1277-1278 bp 1.0 41.5
trnG-UCC-trnT-GGU_p3 GAACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 39.1 47.6 57.3 57.5 1276-1277 bp 1.0 41.5
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGA 38.1 45.0 55.5 56.4 1274-1275 bp 1.0 41.5
trnG-UCC-trnT-GGU_p5 AACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 36.4 47.6 56.2 57.5 1275-1276 bp 1.0 41.5