primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	rps19	1	TTCTGACCTCCGTACTTCGA	TCTTAATCTTTCTATTCAGAATTCAG	20	26	50.0	26.9	57.8	53.5	0.0	8.4	10.736841	275	556	385-385	1.000000	44.0
rps19_p2	rps19	2	TCTGACCTCCGTACTTCGAT	TCTTAATCTTTCTATTCAGAATTCAG	20	26	50.0	26.9	57.6	53.5	0.0	8.4	10.962241	275	556	384-384	1.000000	44.0
rps19_p3	rps19	3	CCTCCGTACTTCGATCGAGA	TCTTAATCTTTCTATTCAGAATTCAG	20	26	55.0	26.9	58.4	53.5	18.5	8.4	10.967549	275	556	379-379	1.000000	44.0
rps19_p4	rps19	4	TTCTGACCTCCGTACTTCGA	CTTCTTAATCTTTCTATTCAGAATTCA	20	27	50.0	25.9	57.8	54.2	0.0	8.4	11.036846	275	556	387-387	1.000000	44.0
rps19_p5	rps19	5	TTCTGACCTCCGTACTTCGA	TTCTTAATCTTTCTATTCAGAATTCAG	20	27	50.0	25.9	57.8	54.2	0.0	8.4	11.036846	275	556	386-386	1.000000	44.0
rps19-psbA_p1	rps19-psbA	1	CAAATTGGGGGAATTCGTGC	TGGAAGTAATGCACGAACGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.166586	557	686	289-289	0.500000	58.7
rps19-psbA_p2	rps19-psbA	2	GCAAATTGGGGGAATTCGTG	TGGAAGTAATGCACGAACGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.166586	557	686	290-290	0.500000	58.7
rps19-psbA_p3	rps19-psbA	3	CAAATTGGGGGAATTCGTGC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.181603	557	686	309-309	0.500000	58.5
rps19-psbA_p4	rps19-psbA	4	GCAAATTGGGGGAATTCGTG	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.181603	557	686	310-310	0.500000	58.5
rps19-psbA_p5	rps19-psbA	5	CAAATTGGGGGAATTCGTGC	ATCAACCGTGCTAACCTTGG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.187535	557	686	311-311	0.500000	58.5
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	ACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	21	21	38.1	47.6	55.5	57.5	0.0	10.2	5.002555	14190	15385	1274-1275	1.000000	41.5
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	AGAACGAATCACACTTTTACCAC	TGACTTACGTCTTAGCACGAG	23	21	39.1	47.6	57.3	57.5	0.0	10.2	5.135270	14190	15385	1277-1278	1.000000	41.5
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	GAACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	23	21	39.1	47.6	57.3	57.5	0.0	10.2	5.135270	14190	15385	1276-1277	1.000000	41.5
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	ACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGA	21	20	38.1	45.0	55.5	56.4	0.0	10.2	5.178366	14190	15385	1274-1275	1.000000	41.5
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	AACGAATCACACTTTTACCACT	TGACTTACGTCTTAGCACGAG	22	21	36.4	47.6	56.2	57.5	0.0	10.2	5.243025	14190	15385	1275-1276	1.000000	41.5
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	58.5	55.8	5.4	0.0	2.706630	16565	17368	855-915	1.000000	62.8
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	57.0	55.8	5.4	0.0	3.226784	16565	17368	856-916	1.000000	58.6
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	19	20	52.6	45.0	57.9	55.8	0.0	0.0	3.376082	16565	17368	869-929	1.000000	57.4
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	19	20	52.6	45.0	57.8	55.8	5.4	0.0	3.461033	16565	17368	854-914	1.000000	56.8
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	GCGGGGATTGTAGTTCAATTG	AGAATGAACAGTGCAGTAGC	21	20	47.6	45.0	57.6	55.8	0.0	0.0	3.650138	16565	17368	868-928	1.000000	55.2
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	17474	18220	841-851	1.000000	69.3
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	17474	18220	840-850	1.000000	69.3
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	17474	18220	899-909	1.000000	68.9
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	21	20	42.9	55.0	57.6	57.8	0.0	0.0	1.583671	17474	18220	839-849	1.000000	68.8
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	21	20	42.9	60.0	57.6	58.2	0.0	6.7	1.624352	17474	18220	836-846	1.000000	68.5
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	18311	19031	829-1042	1.000000	72.1
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	18311	19031	828-1041	1.000000	72.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	18311	19031	770-983	1.000000	71.9
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	18311	19031	831-1044	1.000000	71.6
petN-trnC-GCA_p5	petN-trnC-GCA	5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	18311	19031	830-1043	1.000000	71.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	46648	47269	723-814	1.000000	80.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	46648	47269	715-806	1.000000	80.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	46648	47269	693-784	1.000000	80.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	46648	47269	713-804	1.000000	79.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	46648	47269	722-813	1.000000	78.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	GGATTTGGAGAAGTGCCGAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096018	48309	48898	786-797	1.000000	82.4
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGCTTTGATCACCGAGGAAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096018	48309	48898	1033-1044	1.000000	81.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TTTACGCTCCCAATGTGCTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.147609	48309	48898	941-952	1.000000	81.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	GGGATTGGGATTCCATTGCT	20	20	50.0	50.0	58.1	57.9	0.0	4.8	0.144494	48309	48898	990-1001	1.000000	81.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	GGAGGGGCTCTTTTGTGTAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.167238	48309	48898	1192-1203	1.000000	80.7
rbcL-psaI_p1	rbcL-psaI	1	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.0	57.9	0.0	26.9	0.115800	56470	58145	1929-2010	1.000000	84.0
rbcL-psaI_p2	rbcL-psaI	2	GATCATATCCACGCCGGTAC	GTCATTGCAATTGCCGGAAA	20	20	55.0	45.0	58.0	57.9	1.9	26.9	0.085243	56470	58145	2131-2212	1.000000	83.8
rbcL-psaI_p3	rbcL-psaI	3	ATATCCACGCCGGTACAGTA	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.0	57.9	1.9	26.9	0.091940	56470	58145	2127-2208	1.000000	83.7
rbcL-psaI_p4	rbcL-psaI	4	GATCATATCCACGCCGGTAC	CGCATCCATAGCCGTTTTTC	20	20	55.0	50.0	58.0	57.9	1.9	0.0	0.113153	56470	58145	2263-2344	1.000000	83.3
rbcL-psaI_p5	rbcL-psaI	5	GATCATATCCACGCCGGTAC	TGAAATCGCCTTCAGCAACT	20	20	55.0	45.0	58.0	58.1	1.9	0.0	0.111611	56470	58145	2421-2502	1.000000	83.1
rps18_p1	rps18	1	AGACTACTTCTTCTGGATCCT	GGTAGGAATCAACGAGCTGG	21	20	42.9	55.0	54.8	58.1	14.2	0.0	4.226283	66411	66923	674-675	1.000000	46.9
rps18_p2	rps18	2	AGACTACTTCTTCTGGATCCT	AGGGAACTCCGGGAAGATAC	21	20	42.9	55.0	54.8	58.2	14.2	0.0	4.376790	66411	66923	712-713	1.000000	45.7
rps18_p3	rps18	3	AAGACTACTTCTTCTGGATCCT	GGTAGGAATCAACGAGCTGG	22	20	40.9	55.0	55.6	58.1	14.2	0.0	4.416502	66411	66923	675-676	1.000000	45.4
rps18_p4	rps18	4	AAGACTACTTCTTCTGGATCCT	AGGGAACTCCGGGAAGATAC	22	20	40.9	55.0	55.6	58.2	14.2	0.0	4.567009	66411	66923	713-714	1.000000	44.1
rps18_p5	rps18	5	AGACTACTTCTTCTGGATCCT	TAGGTCTGGCCCTTTCGAAT	21	20	42.9	50.0	54.8	58.4	14.2	0.0	4.587549	66411	66923	632-633	1.000000	44.1
psbT-psbN_p1	psbT-psbN	1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026579	71368	71415	217-217	1.000000	81.6
psbT-psbN_p2	psbT-psbN	2	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	71368	71415	212-212	1.000000	81.6
psbT-psbN_p3	psbT-psbN	3	AAGGTTCCACCAACTCCAAC	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082822	71368	71415	197-197	1.000000	81.4
psbT-psbN_p4	psbT-psbN	4	CCACCTAAGGTTCCACCAAC	TCGCCATCTCCATATCTGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.109819	71368	71415	203-203	1.000000	81.1
psbT-psbN_p5	psbT-psbN	5	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACACGGAGA	20	20	50.0	50.0	58.0	57.6	0.0	3.3	0.374910	71368	71415	128-128	1.000000	80.1
rpl16_p1	rpl16	1	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAGA	20	21	50.0	42.9	58.7	57.5	6.3	0.0	2.199712	78406	79866	1513-1601	1.000000	66.6
rpl16_p2	rpl16	2	CCAGAAAGAAAAACCGGGCA	TCCTGAAATCCCTGCAAAAGA	20	21	50.0	42.9	59.0	57.5	6.3	0.0	2.487387	78406	79866	1512-1600	1.000000	64.3
rpl16_p3	rpl16	3	CAGAAAGAAAAACCGGGCAT	TCCTGAAATCCCTGCAAAAGA	20	21	45.0	42.9	56.9	57.5	6.3	0.0	2.631193	78406	79866	1511-1599	1.000000	63.1
rpl16_p4	rpl16	4	TCCAGAAAGAAAAACCGGGC	CCTGAAATCCCTGCAAAAGA	20	20	50.0	45.0	58.7	55.9	6.3	0.0	2.765439	78406	79866	1512-1600	1.000000	62.0
rpl16_p5	rpl16	5	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAG	20	20	50.0	45.0	58.7	55.9	6.3	0.0	2.765439	78406	79866	1513-1601	1.000000	62.0
trnH-GUG_p1	trnH-GUG	1	TCGAAGTACGGAGGTCAGAA	TCTTCGTCGCCGTAAGTAAA	20	20	50.0	45.0	57.8	57.0	0.0	0.0	1.177007	81736	81810	228-228	1.000000	71.3
trnH-GUG_p2	trnH-GUG	2	TCGAAGTACGGAGGTCAGAA	TTCTTCGTCGCCGTAAGTAA	20	20	50.0	45.0	57.8	57.0	0.0	0.0	1.177007	81736	81810	229-229	1.000000	71.3
trnH-GUG_p3	trnH-GUG	3	TCGAAGTACGGAGGTCAGAA	CGTCGCCGTAAGTAAATACG	20	20	50.0	50.0	57.8	56.9	0.0	0.2	1.335485	81736	81810	224-224	1.000000	70.0
trnH-GUG_p4	trnH-GUG	4	TCGAAGTACGGAGGTCAGAA	GTTTTATTCTTCGTCGCCGT	20	20	50.0	45.0	57.8	56.9	0.0	0.0	1.337476	81736	81810	235-235	1.000000	70.0
trnH-GUG_p5	trnH-GUG	5	TCGAAGTACGGAGGTCAGAA	ATTCTTCGTCGCCGTAAGTA	20	20	50.0	45.0	57.8	56.8	0.0	0.0	1.401008	81736	81810	230-230	1.000000	69.5
rpl2_p1	rpl2	1	TCGCCCATCGCATTATTGC	CAAAACACCTATCCCGAGCA	19	20	52.6	50.0	59.1	57.9	0.0	0.0	2.159442	81871	83268	1533-1533	1.000000	64.1
rpl2_p2	rpl2	2	TCGCCCATCGCATTATTGC	AAAACACCTATCCCGAGCAC	19	20	52.6	50.0	59.1	57.9	0.0	0.0	2.160916	81871	83268	1532-1532	1.000000	64.1
rpl2_p3	rpl2	3	TCGCCCATCGCATTATTGC	ACAAAACACCTATCCCGAGC	19	20	52.6	50.0	59.1	57.9	0.0	0.0	2.160916	81871	83268	1534-1534	1.000000	64.1
rpl2_p4	rpl2	4	TCGCCCATCGCATTATTGC	CCGTAGACAGGCAAGTGAAA	19	20	52.6	50.0	59.1	57.8	0.0	0.0	2.215310	81871	83268	1501-1501	1.000000	63.7
rpl2_p5	rpl2	5	TCGCCCATCGCATTATTGC	GAACCGTAGACAGGCAAGTG	19	20	52.6	55.0	59.1	58.6	0.0	0.0	2.622029	81871	83268	1504-1504	1.000000	60.4
ndhH_p1	ndhH	1	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	58.0	58.0	34.6	0.0	0.054997	102653	102904	639-646	1.000000	85.3
ndhH_p2	ndhH	2	ATCCCGTGAAATTCTCGAGC	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	58.1	58.0	17.5	0.0	0.100387	102653	102904	1140-1147	1.000000	82.5
ndhH_p3	ndhH	3	CTTTTGGGAAAACGTCGACG	AACACTTTTGCCTATCCGCA	20	20	50.0	45.0	58.0	58.1	34.6	0.0	0.108999	102653	102904	1147-1154	1.000000	82.4
ndhH_p4	ndhH	4	CTTTTGGGAAAACGTCGACG	ACACTTTTGCCTATCCGCAT	20	20	50.0	45.0	58.0	57.9	34.6	0.0	0.142056	102653	102904	1146-1153	1.000000	82.1
ndhH_p5	ndhH	5	ATCCCGTGAAATTCTCGAGC	AACACTTTTGCCTATCCGCA	20	20	50.0	45.0	58.1	58.1	17.5	0.0	0.154388	102653	102904	1648-1655	1.000000	81.1
ndhH-ndhF_p1	ndhH-ndhF	1	AAAATCGCGGAAAACCGAAC	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.0	58.0	6.1	0.0	0.082426	102905	102919	239-246	1.000000	91.3
ndhH-ndhF_p2	ndhH-ndhF	2	ACCCATCAATGCATGGTGTT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.100335	102905	102919	340-347	1.000000	89.9
ndhH-ndhF_p3	ndhH-ndhF	3	AACACCCATCAATGCATGGT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.100335	102905	102919	343-350	1.000000	89.8
ndhH-ndhF_p4	ndhH-ndhF	4	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	58.0	58.0	34.6	0.0	0.054997	102905	102919	639-646	1.000000	85.3
ndhH-ndhF_p5	ndhH-ndhF	5	CTTTTGGGAAAACGTCGACG	AACACTTTTGCCTATCCGCA	20	20	50.0	45.0	58.0	58.1	34.6	0.0	0.108999	102905	102919	1147-1154	1.000000	82.4
ndhF_p1	ndhF	1	AAAATCGCGGAAAACCGAAC	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	58.0	58.4	6.1	0.0	0.455990	102920	105139	2938-2944	1.000000	77.8
ndhF_p2	ndhF	2	AATCGCGGAAAACCGAACTA	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.9	58.4	6.1	0.0	0.498442	102920	105139	2936-2942	1.000000	77.5
ndhF_p3	ndhF	3	TCGCGGAAAACCGAACTATT	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.9	58.4	6.1	0.0	0.498442	102920	105139	2934-2940	1.000000	77.5
ndhF_p4	ndhF	4	AAAATCGCGGAAAACCGAAC	ATGGTACGGAAGCCTATCCC	20	20	45.0	55.0	58.0	58.7	6.1	0.0	0.691648	102920	105139	2944-2950	1.000000	76.0
ndhF_p5	ndhF	5	ATCGCGGAAAACCGAACTAT	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.7	58.4	6.1	0.0	0.720027	102920	105139	2935-2941	1.000000	75.7