Hotspot

ycf3

LSC · 43949-45942 · 1994 bp

Back to Prunus

Pi 0.00632
MarkerSeek score 45.50
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1994
variable_sites
125
pi
0.00632
unique_haplotype_count
61
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 CCCAGAGAAATGCTCCGTTT GGTGTTTCCGCCCTTTCTAA 50.0 50.0 58.2 58.1 2533-2913 bp 0.975 86.7
ycf3_p2 CCCAGAGAAATGCTCCGTTT AAAAATCCTTCCTCCGAGGC 50.0 50.0 58.2 57.9 2626-2966 bp 0.941667 85.2
ycf3_p3 GATCTGGAAGTTGATCCGGG GGTGTTTCCGCCCTTTCTAA 55.0 50.0 57.8 58.1 2942-2993 bp 0.825 77.9
ycf3_p4 CCCAGAGAAATGCTCCGTTT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.2 58.2 2828-2871 bp 0.8 76.7
ycf3_p5 CCCAGAGAAATGCTCCGTTT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.2 57.8 2778-2821 bp 0.8 76.5