Hotspot

ndhF

SSC · 112432-114675 · 2244 bp

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Pi 0.00932
MarkerSeek score 51.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2244
variable_sites
190
pi
0.00932
unique_haplotype_count
92
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGAAGGGGACTAAACAGGA TGGGTCAAGTTATGCGCTTT 50.0 45.0 58.0 58.1 2696-2845 bp 0.816667 79.2
ndhF_p2 ACAGGAACAAGAGGGATCCA TGGGTCAAGTTATGCGCTTT 50.0 45.0 58.0 58.1 2682-2831 bp 0.816667 79.0
ndhF_p3 AAGAGGGATCCACCGAAGAA TGGGTCAAGTTATGCGCTTT 50.0 45.0 58.0 58.1 2674-2823 bp 0.816667 79.0
ndhF_p4 GCAGAATACCGTCACCCATT TGGGTCAAGTTATGCGCTTT 50.0 45.0 58.0 58.1 2785-2934 bp 0.816667 78.9
ndhF_p5 ACGAAGGGGACTAAACAGGA ATGGGTCAAGTTATGCGCTT 50.0 45.0 58.0 57.9 2697-2846 bp 0.766667 76.9