Hotspot

ndhF-rpl32

SSC · 114676-115576 · 901 bp

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Pi 0.0162
MarkerSeek score 60.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
901
variable_sites
139
pi
0.0162
unique_haplotype_count
75
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGAAATTGGGCGGATTTTG TTCAATGCTGCCCGATATCC 50.0 50.0 58.0 58.1 1697-1773 bp 1.0 91.0
ndhF-rpl32_p2 GGAAGGCGCATAAGGTAACA TTCAATGCTGCCCGATATCC 50.0 50.0 58.0 58.1 1656-1732 bp 1.0 90.9
ndhF-rpl32_p3 GGGAAATTGGGCGGATTTTG AGTCGATGGGGAAAGTGAGA 50.0 50.0 58.0 58.1 2009-2320 bp 0.941667 89.0
ndhF-rpl32_p4 GGAAGGCGCATAAGGTAACA AGTCGATGGGGAAAGTGAGA 50.0 50.0 58.0 58.1 1968-2279 bp 0.941667 88.9
ndhF-rpl32_p5 GAAATTGGGCGGATTTTGCA AGTCGATGGGGAAAGTGAGA 45.0 50.0 57.9 58.1 2007-2318 bp 0.941667 88.4