Hotspot

rpl33-rps18

LSC · 69671-69842 · 172 bp

Back to Prunus

Pi 0.0397
MarkerSeek score 61.10
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
172
variable_sites
26
pi
0.0397
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 GCCTGGTCGATTGGAATTGA TCAATTCGATCCCCCGATTG 50.0 50.0 58.2 57.7 334-347 bp 1.0 84.1
rpl33-rps18_p2 ACAATACGCCTGGTCGATTG TCAATTCGATCCCCCGATTG 50.0 50.0 58.3 57.7 341-354 bp 1.0 83.1
rpl33-rps18_p3 CACAATACGCCTGGTCGATT TCAATTCGATCCCCCGATTG 50.0 50.0 58.3 57.7 342-355 bp 1.0 83.1
rpl33-rps18_p4 AATCGACACAATACGCCTGG TCAATTCGATCCCCCGATTG 50.0 50.0 58.3 57.7 348-361 bp 0.95 80.9
rpl33-rps18_p5 GCCTGGTCGATTGGAATTGA ATTCGATCCCCCGATTGGAT 50.0 50.0 58.2 58.6 331-344 bp 0.991667 80.5