Hotspot

psbE-petL

LSC · 66419-67699 · 1281 bp

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Pi 0.0116
MarkerSeek score 62.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1281
variable_sites
137
pi
0.0116
unique_haplotype_count
79
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC TCAAAGCTGCTAGTAGAAAACCA 50.0 39.1 57.8 58.0 1335-1452 bp 1.0 66.5
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC TCAAAGCTGCTAGTAGAAAACCA 55.0 39.1 58.2 58.0 1330-1447 bp 1.0 66.2
psbE-petL_p3 ACGAATAACCAACCCGCAAT TCAAAGCTGCTAGTAGAAAACCA 45.0 39.1 58.2 58.0 1421-1538 bp 0.991667 65.9
psbE-petL_p4 TGCTGACGAATAACCAACCC TCAAAGCTGCTAGTAGAAAACCA 50.0 39.1 58.2 58.0 1426-1543 bp 0.991667 65.8
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT TCAAAGCTGCTAGTAGAAAACCA 50.0 39.1 58.4 58.0 1328-1445 bp 1.0 64.9