Hotspot

trnT-GGU-psbD

LSC · 33066-34283 · 1218 bp

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Pi 0.0119
MarkerSeek score 68.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1218
variable_sites
139
pi
0.0119
unique_haplotype_count
71
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1367-1496 bp 0.991667 90.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1370-1499 bp 0.991667 90.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1369-1498 bp 0.991667 90.7
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1372-1501 bp 0.991667 90.5
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGCCAGACCAACCTACAAAA 50.0 50.0 58.3 58.0 1344-1473 bp 1.0 89.7