Hotspot

petN-psbM

LSC · 29307-30521 · 1215 bp

Back to Prunus

Pi 0.0145
MarkerSeek score 56.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1215
variable_sites
163
pi
0.0145
unique_haplotype_count
68
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AAAACAGTCAGCCAAGGTGA 50.0 45.0 58.0 57.6 1263-1367 bp 1.0 88.3
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG AAACAGTCAGCCAAGGTGAT 50.0 45.0 58.0 57.3 1262-1366 bp 1.0 86.4
petN-psbM_p3 AGTAAATCTCGCTTGGGCTG AACAGTCAGCCAAGGTGATT 50.0 45.0 58.0 57.3 1261-1365 bp 1.0 86.4
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT AAAACAGTCAGCCAAGGTGA 50.0 45.0 57.6 57.6 1203-1307 bp 1.0 85.7
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT AACAGTCAGCCAAGGTGATT 50.0 45.0 57.6 57.3 1201-1305 bp 1.0 83.9