Hotspot

trnR-UCU-atpA

LSC · 9611-10042 · 432 bp

Back to Prunus

Pi 0.0259
MarkerSeek score 67.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
432
variable_sites
110
pi
0.0259
unique_haplotype_count
73
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TGGAATGAAAAGCGTCCATTG AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 383-688 bp 0.966667 75.1
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 382-687 bp 0.966667 75.1
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 47.6 50.0 57.3 58.4 322-627 bp 0.966667 74.1
trnR-UCU-atpA_p4 TGGATAGGACAGAGGTCTTCT AGACATTTACCGACGAAGCG 47.6 50.0 56.9 58.4 357-662 bp 0.966667 71.3
trnR-UCU-atpA_p5 TGGAATGAAAAGCGTCCATT AGACATTTACCGACGAAGCG 40.0 50.0 55.9 58.4 383-688 bp 0.966667 71.0