Hotspot

rps16-trnQ-UUG

LSC · 6367-7051 · 685 bp

Back to Prunus

Pi 0.0128
MarkerSeek score 64.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
685
variable_sites
106
pi
0.0128
unique_haplotype_count
66
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 566-975 bp 1.0 91.2
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 564-973 bp 1.0 91.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 548-957 bp 1.0 90.3
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 559-968 bp 1.0 89.8
rps16-trnQ-UUG_p5 CAGCGGATCATGTCCTTCAA GTGTCGACTAGAAATGGGGC 50.0 55.0 58.0 58.3 628-1037 bp 1.0 89.0