Hotspot

ycf1

SSC · 121245-126791 · 5547 bp

Back to Prunella

Pi 0.00252

MarkerSeek score 39.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 14
pi: 0.00252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGTTAATTTACAGATTAATTGAGCA	ATTGCATCTCCGAGTTGGAC	26.9	50.0	55.5	58.0	232 bp	1.0	41.0
ycf1_p2	GGTTAATTTACAGATTAATTGAGCA	ATTGCATCTCCGAGTTGGAC	28.0	50.0	54.2	58.0	231 bp	1.0	41.0
ycf1_p3	AAGGTTAATTTACAGATTAATTGAGCA	ATTGCATCTCCGAGTTGGAC	25.9	50.0	56.1	58.0	233 bp	1.0	41.0
ycf1_p4	AGGTTAATTTACAGATTAATTGAGCAT	ATTGCATCTCCGAGTTGGAC	25.9	50.0	55.9	58.0	232 bp	1.0	41.0
ycf1_p5	AGGTTAATTTACAGATTAATTGAGC	ATTGCATCTCCGAGTTGGAC	28.0	50.0	53.9	58.0	232 bp	1.0	41.0