Hotspot

ndhF

SSC · 108264-110495 · 2232 bp

Back to Prunella

Pi 0.00314

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 7
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCGTTTCTGAAGAGTC	ACTCCTTTAAAAAGAAGTAAAATCCGA	50.0	29.6	56.9	57.7	2358 bp	1.0	40.2
ndhF_p2	GACCCGTTTCTGAAGAGTCT	ACTCCTTTAAAAAGAAGTAAAATCCGA	50.0	29.6	56.9	57.7	2357 bp	1.0	40.2
ndhF_p3	AGACCCGTTTCTGAAGAGTC	ACTCCTTTAAAAAGAAGTAAAATCCG	50.0	30.8	56.9	56.5	2358 bp	1.0	40.2
ndhF_p4	GACCCGTTTCTGAAGAGTCT	ACTCCTTTAAAAAGAAGTAAAATCCG	50.0	30.8	56.9	56.5	2357 bp	1.0	40.2
ndhF_p5	GACCCGTTTCTGAAGAGTCT	GACTCCTTTAAAAAGAAGTAAAATCCG	50.0	33.3	56.9	57.4	2358 bp	1.0	40.2