Hotspot

matK

LSC · 1962-3488 · 1527 bp

Back to Prunella

Pi 0.00262

MarkerSeek score 60.20

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 4
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCTCTTCATCCATTTTCTTTACGA	AGTTTACTTGTCCCCGAGGT	33.3	50.0	56.1	58.3	1679 bp	1.0	40.2
matK_p2	TCTCTTCATCCATTTTCTTTACGA	CCCCCGAATCTTCTACCCTT	33.3	55.0	56.1	58.5	1601 bp	1.0	40.2
matK_p3	TCTCTTCATCCATTTTCTTTACGAA	AGTTTACTTGTCCCCGAGGT	32.0	50.0	56.8	58.3	1679 bp	1.0	40.2
matK_p4	CTCTTCATCCATTTTCTTTACGA	AGTTTACTTGTCCCCGAGGT	34.8	50.0	54.7	58.3	1678 bp	1.0	40.2
matK_p5	TCTCTTCATCCATTTTCTTTACG	AGTTTACTTGTCCCCGAGGT	34.8	50.0	54.7	58.3	1679 bp	1.0	40.2