Hotspot

rps15-ycf1

SSC · 120809-121244 · 436 bp

Back to Prunella

Pi 0.00688

MarkerSeek score 55.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 436
variable_sites: 3
pi: 0.00688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGCCTCCTTATCTTAGTGGTGA	TGGCAGTCGTTTCAGTATGT	45.5	45.0	58.0	57.2	604 bp	1.0	57.7
rps15-ycf1_p2	AGCCTCCTTATCTTAGTGGTGA	TGGCAGTCGTTTCAGTATGTT	45.5	42.9	58.0	57.9	604 bp	1.0	55.4
rps15-ycf1_p3	AGCCTCCTTATCTTAGTGGTGA	CCGCGATTGAAATTCTGTGA	45.5	45.0	58.0	56.8	567 bp	1.0	54.9
rps15-ycf1_p4	AGCCTCCTTATCTTAGTGGTGA	TCCGCGATTGAAATTCTGTG	45.5	45.0	58.0	56.8	568 bp	1.0	54.9
rps15-ycf1_p5	GCCTCCTTATCTTAGTGGTGA	TGGCAGTCGTTTCAGTATGT	47.6	45.0	56.5	57.2	603 bp	1.0	54.0