Hotspot

ycf3-trnS-GGA

LSC · 43790-44631 · 842 bp

Back to Prunella

Pi 0.00356
MarkerSeek score 56.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
842
variable_sites
3
pi
0.00356
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1014 bp 1.0 72.5
ycf3-trnS-GGA_p2 TGCCTCCTTTTCTCCTGAAG ATTAAGTGCAGGTGCGGAAA 50.0 45.0 57.1 58.1 1048 bp 1.0 72.5
ycf3-trnS-GGA_p3 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1013 bp 1.0 72.5
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT ATTAAGTGCAGGTGCGGAAA 50.0 45.0 57.1 58.1 1047 bp 1.0 72.5
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 985 bp 1.0 71.8