Hotspot

trnT-GGU-psbD

LSC · 30682-32016 · 1335 bp

Back to Prunella

Pi 0.003

MarkerSeek score 60.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1335
variable_sites: 4
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1495-1499 bp	1.0	78.8
trnT-GGU-psbD_p2	GTGACTCGTTCGGGAATTCA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1538-1542 bp	1.0	78.7
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1498-1502 bp	1.0	78.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1485-1489 bp	1.0	77.7
trnT-GGU-psbD_p5	GTGACTCGTTCGGGAATTCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1528-1532 bp	1.0	77.6