Hotspot

petN-psbM

LSC · 28001-29020 · 1020 bp

Back to Prunella

Pi 0.00392

MarkerSeek score 61.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 4
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1109-1113 bp	1.0	59.4
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1107-1111 bp	1.0	59.4
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1116-1120 bp	1.0	59.2
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1114-1118 bp	1.0	59.2
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	55.0	42.9	57.9	56.4	1106-1110 bp	1.0	58.8