Hotspot

rps16-trnQ-UUG

LSC · 5845-6635 · 791 bp

Back to Prunella

Pi 0.00637

MarkerSeek score 58.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 5
pi: 0.00637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GTCCTGTCATTCGGAGGTTC	45.0	55.0	58.1	58.0	871-877 bp	1.0	79.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	858-864 bp	1.0	79.3
rps16-trnQ-UUG_p3	AAAAATCCACAACGGGTCGT	GTCCTGTCATTCGGAGGTTC	45.0	55.0	58.3	58.0	897-903 bp	1.0	78.3
rps16-trnQ-UUG_p4	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	884-890 bp	1.0	77.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCCTGTCATT	45.0	50.0	58.1	58.4	880-886 bp	1.0	76.8