Hotspot

ycf1

SSC · 112628-117904 · 5277 bp

Back to Protea

Pi 0.00683
MarkerSeek score 42.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5277
variable_sites
36
pi
0.00683
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACACTACAGATATGATCTTTTATCACA GCCCCAGTATCCATATTGGC 29.6 55.0 56.4 58.2 309 bp 1.0 42.1
ycf1_p2 ACACTACAGATATGATCTTTTATCACA GTATCCATATTGGCCCAGGC 29.6 55.0 56.4 58.2 303 bp 1.0 42.1
ycf1_p3 ACACTACAGATATGATCTTTTATCACA CCCAGTATCCATATTGGCCC 29.6 55.0 56.4 57.5 307 bp 1.0 42.1
ycf1_p4 ACACTACAGATATGATCTTTTATCACA CCCCAGTATCCATATTGGCC 29.6 55.0 56.4 57.5 308 bp 1.0 42.1
ycf1_p5 ACACTACAGATATGATCTTTTATCACA AGAATCAGCCCCAGTATCCA 29.6 50.0 56.4 57.8 316 bp 1.0 42.0