Hotspot

ndhF

SSC · 129998-132226 · 2229 bp

Back to Protea

Pi 0.00763
MarkerSeek score 52.80
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2229
variable_sites
17
pi
0.00763
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GAGCTGGCGAATCGGAAATA TCTGGTTTGAAAAACCTCTTGT 50.0 36.4 58.1 56.3 2317 bp 1.0 50.0
ndhF_p2 AGCTGGCGAATCGGAAATAA TCTGGTTTGAAAAACCTCTTGT 45.0 36.4 57.7 56.3 2316 bp 1.0 48.4
ndhF_p3 AGATAAGAGCTGGCGAATCG TCTGGTTTGAAAAACCTCTTGT 50.0 36.4 57.6 56.3 2323 bp 1.0 47.6
ndhF_p4 GAGCTGGCGAATCGGAAATA TCTGGTTTGAAAAACCTCTTGTT 50.0 34.8 58.1 57.0 2317 bp 1.0 47.6
ndhF_p5 GAGCTGGCGAATCGGAAATA TTCTGGTTTGAAAAACCTCTTGT 50.0 34.8 58.1 57.0 2318 bp 1.0 47.6