Hotspot

trnN-GUU-trnR-ACG

IRa · 133669-134272 · 604 bp

Back to Protea

Pi 0.00662
MarkerSeek score 62.60
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
604
variable_sites
4
pi
0.00662
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 746-752 bp 1.0 78.7
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 783-789 bp 1.0 78.1
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 697-703 bp 1.0 77.5
trnN-GUU-trnR-ACG_p4 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 786-792 bp 1.0 77.1
trnN-GUU-trnR-ACG_p5 CTGGTCGTAGGTTCGAATCC CAGAGGATTAGAGCACGTGG 55.0 55.0 57.8 57.8 698-704 bp 1.0 77.1