Hotspot

trnL-UAG-rpl32

SSC · 127619-128734 · 1116 bp

Back to Protea

Pi 0.00907
MarkerSeek score 50.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1116
variable_sites
10
pi
0.00907
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TTTAGAAGATGCCGCCACTC ATTGGGCAGCAGTAAAAGCT 50.0 45.0 58.0 58.1 1293-1306 bp 1.0 80.7
trnL-UAG-rpl32_p2 AGCAGGTACCAATATTGCGG ATTGGGCAGCAGTAAAAGCT 50.0 45.0 58.0 58.1 1773-1786 bp 1.0 80.3
trnL-UAG-rpl32_p3 GCAGGTACCAATATTGCGGA ATTGGGCAGCAGTAAAAGCT 50.0 45.0 58.0 58.1 1772-1785 bp 1.0 80.3
trnL-UAG-rpl32_p4 TCGAACCGAGATGCTTTAGC ATTGGGCAGCAGTAAAAGCT 50.0 45.0 58.1 58.1 1269-1282 bp 1.0 80.3
trnL-UAG-rpl32_p5 GCCAAAGGAAGATGACCCAA ATTGGGCAGCAGTAAAAGCT 50.0 45.0 58.1 58.1 1941-1954 bp 1.0 79.9