Hotspot

ndhD-ccsA

SSC · 126189-126470 · 282 bp

Back to Protea

Pi 0.0426
MarkerSeek score 62.60
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
282
variable_sites
12
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TTCTCGATGGCTTCTCTTGC CCGCAATATTGGTACCTGCT 50.0 50.0 58.0 58.0 1241 bp 1.0 80.6
ndhD-ccsA_p2 TTCTCGATGGCTTCTCTTGC TCCGCAATATTGGTACCTGC 50.0 50.0 58.0 58.0 1242 bp 1.0 80.5
ndhD-ccsA_p3 CTCGATGGCTTCTCTTGCAT CCGCAATATTGGTACCTGCT 50.0 50.0 58.0 58.0 1239 bp 1.0 80.3
ndhD-ccsA_p4 CTCGATGGCTTCTCTTGCAT TCCGCAATATTGGTACCTGC 50.0 50.0 58.0 58.0 1240 bp 1.0 80.3
ndhD-ccsA_p5 TCAACGGGGCTGTTTTACAA CCGCAATATTGGTACCTGCT 45.0 50.0 57.9 58.0 1399 bp 1.0 80.2