Hotspot

accD-psaI

LSC · 61039-61747 · 709 bp

Back to Protea

Pi 0.00292
MarkerSeek score 63.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
709
variable_sites
2
pi
0.00292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGCTTCACGGTTTCTTTCCC GCCATTGCAATTGCTGGAAA 50.0 45.0 58.4 58.2 802-824 bp 1.0 77.8
accD-psaI_p2 GCTTCACGGTTTCTTTCCCT GCCATTGCAATTGCTGGAAA 50.0 45.0 58.4 58.2 801-823 bp 1.0 77.8
accD-psaI_p3 AGCTTCACGGTTTCTTTCCC AGAAGCCATTGCAATTGCTG 50.0 45.0 58.4 57.6 806-828 bp 1.0 76.3
accD-psaI_p4 GCTTCACGGTTTCTTTCCCT AGAAGCCATTGCAATTGCTG 50.0 45.0 58.4 57.6 805-827 bp 1.0 76.3
accD-psaI_p5 AAAGGCGTTCTGAGTGAGTT GCCATTGCAATTGCTGGAAA 45.0 45.0 57.4 58.2 827-849 bp 1.0 75.9