Hotspot

trnT-UGU-trnL-UAA

LSC · 49420-49964 · 545 bp

Back to Protea

Pi 0.00936
MarkerSeek score 64.90
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
545
variable_sites
5
pi
0.00936
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CTCTAACCGCTGAGCTAAGC CCGTAGCGTCTACCAATTCC 55.0 55.0 58.2 58.2 589-600 bp 1.0 79.7
trnT-UGU-trnL-UAA_p2 CTCTAACCGCTGAGCTAAGC ATCCAATCAAGTCCGTAGCG 55.0 50.0 58.2 57.8 601-612 bp 1.0 79.1
trnT-UGU-trnL-UAA_p3 CTCTAACCGCTGAGCTAAGC AGTCCGTAGCGTCTACCAAT 55.0 50.0 58.2 58.2 592-603 bp 1.0 79.0
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 627-638 bp 1.0 78.9
trnT-UGU-trnL-UAA_p5 CTCTAACCGCTGAGCTAAGC CAATCAAGTCCGTAGCGTCT 55.0 50.0 58.2 57.7 598-609 bp 1.0 78.7