Hotspot

petN-trnT-GGU

LSC · 33665-34436 · 772 bp

Back to Protea

Pi 0.00525
MarkerSeek score 63.00
Primer available yes
Label petN-trnT-GGU

Feature Metadata

label_name
petN-trnT-GGU
feature_type
igs
region
LSC
length_bp
772
variable_sites
4
pi
0.00525
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnT-GGU_p1 CCCCATACTACGAGCGAAAG GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 891-901 bp 1.0 80.1
petN-trnT-GGU_p2 CCCCATACTACGAGCGAAAG TACCGCTGAGTTAAAAGGGC 55.0 50.0 57.9 57.9 856-866 bp 1.0 79.4
petN-trnT-GGU_p3 TCCCCATACTACGAGCGAAA GAACCGATGACTTACGCCTT 50.0 50.0 58.2 58.0 892-902 bp 1.0 79.3
petN-trnT-GGU_p4 TTCCCCATACTACGAGCGAA GAACCGATGACTTACGCCTT 50.0 50.0 58.2 58.0 893-903 bp 1.0 79.3
petN-trnT-GGU_p5 CCCCATACTACGAGCGAAAG CCGATGACTTACGCCTTACC 55.0 55.0 57.9 58.2 888-898 bp 1.0 79.0