Hotspot

ycf2

LSC · 87182-93607 · 6426 bp

Back to Prostanthera

Pi 0.0075

MarkerSeek score 42.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6426
variable_sites: 82
pi: 0.0075
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTCTACCCTTTCCCGGAAGT	CTAGTGGACCTCTCGGTAGG	50.0	60.0	58.0	58.0	1478-1485 bp	1.0	86.3
ycf2_p2	TTCTACCCTTTCCCGGAAGT	GACCTCTCGGTAGGATTCGA	50.0	55.0	58.0	58.0	1472-1479 bp	1.0	86.3
ycf2_p3	GTTGGGCGCTTTAACCATTC	TAGGAAGAAGCCCCCTCAAA	50.0	50.0	58.0	57.9	451-464 bp	1.0	84.8
ycf2_p4	GTTGGGCGCTTTAACCATTC	CTAGTGGACCTCTCGGTAGG	50.0	60.0	58.0	58.0	1061-1074 bp	1.0	82.3
ycf2_p5	GTTGGGCGCTTTAACCATTC	GACCTCTCGGTAGGATTCGA	50.0	55.0	58.0	58.0	1055-1068 bp	1.0	82.3