Hotspot

ccsA-ndhD

SSC · 114825-115056 · 232 bp

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Pi 0.051

MarkerSeek score 78.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 19
pi: 0.051
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AATTGTGGCGTCTGTTGGAT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	58.1	57.4	412-419 bp	1.0	78.7
ccsA-ndhD_p2	ATTGTGGCGTCTGTTGGATT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	58.1	57.4	411-418 bp	1.0	78.7
ccsA-ndhD_p3	TGGCGTCTGTTGGATTTCTT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	57.7	57.4	407-414 bp	1.0	77.3
ccsA-ndhD_p4	TTGTGGCGTCTGTTGGATTT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	58.3	57.4	410-417 bp	1.0	76.9
ccsA-ndhD_p5	AATTCAGCAATTGTGGCGTC	ACCCCGATTTCGTTCTTTCA	45.0	45.0	57.6	57.4	420-427 bp	1.0	76.5