Hotspot

rpl32-trnL

SSC · 113165-113676 · 512 bp

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Pi 0.0387

MarkerSeek score 69.40

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 512
variable_sites: 30
pi: 0.0387
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TTTTTGTGCGACAAACAAAA	TTTAGGAGATGACATGCCGC	30.0	50.0	53.6	58.0	632-638 bp	1.0	49.1
rpl32-trnL_p2	TTTTTGTGCGACAAACAAAA	TCCTAAGAGCAGCGTGTCTA	30.0	50.0	53.6	57.9	577-583 bp	1.0	48.9
rpl32-trnL_p3	TTTTTGTGCGACAAACAAAA	AGCAGCGTGTCTACCAATTT	30.0	45.0	53.6	57.8	570-576 bp	1.0	48.5
rpl32-trnL_p4	TTTTTGTGCGACAAACAAAA	CCTAAGAGCAGCGTGTCTAC	30.0	55.0	53.6	57.8	576-582 bp	1.0	48.3
rpl32-trnL_p5	TTTTTGTGCGACAAACAAAA	CTAAGAGCAGCGTGTCTACC	30.0	55.0	53.6	57.8	575-581 bp	1.0	48.3