Hotspot

rps16-trnQ-UUG

LSC · 6295-7324 · 1030 bp

Back to Prostanthera

Pi 0.0128

MarkerSeek score 62.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 21
pi: 0.0128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1162-1178 bp	1.0	83.5
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1104-1120 bp	1.0	83.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1145-1161 bp	1.0	83.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1087-1103 bp	1.0	82.7
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1163-1179 bp	1.0	82.4