primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6295	7324	1162-1178	1.000000	83.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6295	7324	1104-1120	1.000000	83.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6295	7324	1145-1161	1.000000	83.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6295	7324	1087-1103	1.000000	82.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	58.2	58.0	7.8	0.0	0.217997	6295	7324	1163-1179	1.000000	82.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	AATGCCTCCCTTTCTCCTGA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.420595	45071	45905	1009-1014	1.000000	79.2
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	ATGCCTCCCTTTCTCCTGAA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.420595	45071	45905	1008-1013	1.000000	79.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	AATGCCTCCCTTTCTCCTGA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.477581	45071	45905	1030-1035	1.000000	78.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	ATGCCTCCCTTTCTCCTGAA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.477581	45071	45905	1029-1034	1.000000	78.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	ATGCCTCCCTTTCTCCTGAA	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.3	57.8	0.0	5.6	0.518896	45071	45905	979-984	1.000000	78.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATAGGAATAAGACTTGA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.3	57.8	0.0	0.0	5.853835	51347	52397	1154-1167	1.000000	46.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAAGACTTGA	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	56.3	57.5	0.0	0.0	6.197608	51347	52397	1153-1166	1.000000	46.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAAATAGGAATAAGACTTG	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	54.9	57.8	0.0	0.0	6.253715	51347	52397	1154-1167	1.000000	46.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAAGACTTGA	GTTCGAGTCCGTATAGCCCT	24	20	33.3	55.0	56.3	58.7	0.0	0.0	6.339901	51347	52397	1142-1155	1.000000	46.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAAGACTTGAT	AGAAGGTCTACGGTTCGAGT	25	20	32.0	50.0	56.8	57.8	0.0	0.0	6.395612	51347	52397	1154-1167	1.000000	46.3
rbcL-accD_p1	rbcL-accD	1	TCATGGTATGCACTTCCGTG	TCACTATCACTGCTGTTCGC	20	20	50.0	50.0	58.0	58.0	15.5	0.0	0.026473	57663	58345	1791-1796	0.500000	61.4
rbcL-accD_p2	rbcL-accD	2	TTGAATGCTACTGCGGGTAC	TCACTATCACTGCTGTTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022250	57663	58345	1991-1996	0.500000	61.3
rbcL-accD_p3	rbcL-accD	3	TCTTCACATTCACCGTGCAA	TCACTATCACTGCTGTTCGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.043292	57663	58345	1839-1844	0.500000	61.2
rbcL-accD_p4	rbcL-accD	4	TTCTTCACATTCACCGTGCA	TCACTATCACTGCTGTTCGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.043292	57663	58345	1840-1845	0.500000	61.2
rbcL-accD_p5	rbcL-accD	5	AATGCTACTGCGGGTACATG	TCACTATCACTGCTGTTCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	57663	58345	1988-1993	0.500000	61.0
accD_p1	accD	1	AGGGGGCAAGAAAACTCT	AAATAATGGAACTTGGCGCG	18	20	50.0	45.0	55.5	57.4	0.0	0.0	5.040573	58346	59857	1531-1552	1.000000	44.8
accD_p2	accD	2	AGGGGGCAAGAAAACTCT	AAAATAATGGAACTTGGCGCG	18	21	50.0	42.9	55.5	58.1	0.0	0.0	5.605725	58346	59857	1532-1553	1.000000	44.8
accD_p3	accD	3	AGGGGGCAAGAAAACTCT	AATAATGGAACTTGGCGCGC	18	20	50.0	50.0	55.5	59.9	0.0	25.9	6.380072	58346	59857	1530-1551	1.000000	44.7
accD_p4	accD	4	AGGGGGCAAGAAAACTCT	ATAATGGAACTTGGCGCGC	18	19	50.0	52.6	55.5	59.3	0.0	25.9	6.753108	58346	59857	1529-1550	1.000000	44.7
accD_p5	accD	5	AGGGGGCAAGAAAACTCT	TAAAATAATGGAACTTGGCGCG	18	22	50.0	40.9	55.5	58.0	0.0	0.0	6.503039	58346	59857	1533-1554	0.750000	33.0
petB-petD_p1	petB-petD	1	TGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	20	20	40.0	50.0	55.4	57.7	0.0	0.0	2.957644	77249	77434	254-254	1.000000	63.0
petB-petD_p2	petB-petD	2	TGCACTTTCCAATGATACGT	AGTTCAAGTCACACACTCCC	20	20	40.0	50.0	55.4	57.4	0.0	0.0	3.254125	77249	77434	255-255	1.000000	60.6
petB-petD_p3	petB-petD	3	ATGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	21	20	38.1	50.0	55.9	57.7	0.0	0.0	3.381568	77249	77434	255-255	1.000000	59.5
petB-petD_p4	petB-petD	4	AATGCACTTTCCAATGATACGT	GTTCAAGTCACACACTCCCA	22	20	36.4	50.0	56.7	57.7	0.0	0.0	3.643482	77249	77434	256-256	1.000000	57.4
petB-petD_p5	petB-petD	5	ATGCACTTTCCAATGATACGT	AGTTCAAGTCACACACTCCC	21	20	38.1	50.0	55.9	57.4	0.0	0.0	3.678048	77249	77434	256-256	1.000000	57.1
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	TCGACCCAATGCTTTAGTTCT	GAATGGTTAAAGCGCCCAAC	21	20	42.9	50.0	57.3	58.0	0.0	2.3	1.705031	86826	87019	363-377	1.000000	78.4
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	CGACCCAATGCTTTAGTTCTG	GAATGGTTAAAGCGCCCAAC	21	20	47.6	50.0	57.3	58.0	0.0	2.3	1.756300	86826	87019	362-376	1.000000	77.9
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	TCGACCCAATGCTTTAGTTCT	CGCTAAGCATCCCTAAGCAT	21	20	42.9	50.0	57.3	57.8	0.0	0.0	1.871708	86826	87019	391-405	1.000000	77.0
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	CGACCCAATGCTTTAGTTCTG	CGCTAAGCATCCCTAAGCAT	21	20	47.6	50.0	57.3	57.8	0.0	0.0	1.922977	86826	87019	390-404	1.000000	76.6
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	TTCGACCCAATGCTTTAGTTCT	GAATGGTTAAAGCGCCCAAC	22	20	40.9	50.0	58.0	58.0	0.0	2.3	2.025073	86826	87019	364-378	1.000000	75.8
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	TTCTACCCTTTCCCGGAAGT	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.084913	87094	87181	746-753	1.000000	89.6
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	TTCTACCCTTTCCCGGAAGT	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	58.0	57.9	0.7	0.0	0.088183	87094	87181	868-875	1.000000	89.2
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.059678	87094	87181	329-342	1.000000	86.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.062948	87094	87181	451-464	1.000000	84.8
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GTTGGGCGCTTTAACCATTC	GGTATAGGAAGAAGCCCCCT	20	20	50.0	55.0	58.0	57.9	7.6	0.0	0.129140	87094	87181	455-468	1.000000	84.2
ycf2_p1	ycf2	1	TTCTACCCTTTCCCGGAAGT	CTAGTGGACCTCTCGGTAGG	20	20	50.0	60.0	58.0	58.0	0.7	0.0	0.064345	87183	87432	1478-1485	1.000000	86.3
ycf2_p2	ycf2	2	TTCTACCCTTTCCCGGAAGT	GACCTCTCGGTAGGATTCGA	20	20	50.0	55.0	58.0	58.0	0.7	10.5	0.069884	87183	87432	1472-1479	1.000000	86.3
ycf2_p3	ycf2	3	GTTGGGCGCTTTAACCATTC	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.062948	87183	87432	451-464	1.000000	84.8
ycf2_p4	ycf2	4	GTTGGGCGCTTTAACCATTC	CTAGTGGACCTCTCGGTAGG	20	20	50.0	60.0	58.0	58.0	7.6	0.0	0.039109	87183	87432	1061-1074	1.000000	82.3
ycf2_p5	ycf2	5	GTTGGGCGCTTTAACCATTC	GACCTCTCGGTAGGATTCGA	20	20	50.0	55.0	58.0	58.0	7.6	10.5	0.044648	87183	87432	1055-1068	1.000000	82.3
ndhF-rpl32_p1	ndhF-rpl32	1	GAAAGGCAAAATCCACGCAT	TGATGCAGAAGTACGCTTTT	20	20	45.0	40.0	57.6	55.7	0.0	0.0	2.682486	112491	113002	584-589	1.000000	63.0
ndhF-rpl32_p2	ndhF-rpl32	2	GAAAGGCAAAATCCACGCAT	TTGATGCAGAAGTACGCTTT	20	20	45.0	40.0	57.6	55.7	0.0	0.0	2.682486	112491	113002	585-590	1.000000	63.0
ndhF-rpl32_p3	ndhF-rpl32	3	GAAAGGCAAAATCCACGCAT	TTTGATGCAGAAGTACGCTT	20	20	45.0	40.0	57.6	55.7	0.0	0.0	2.682486	112491	113002	586-591	1.000000	63.0
ndhF-rpl32_p4	ndhF-rpl32	4	GAAAGGCAAAATCCACGCAT	TTTTGATGCAGAAGTACGCT	20	20	45.0	40.0	57.6	55.7	0.0	0.0	2.682486	112491	113002	587-592	1.000000	63.0
ndhF-rpl32_p5	ndhF-rpl32	5	AACGAAAGGCAAAATCCACG	TGATGCAGAAGTACGCTTTT	20	20	45.0	40.0	57.6	55.7	0.0	0.0	2.729529	112491	113002	587-592	1.000000	62.6
rpl32-trnL_p1	rpl32-trnL	1	TTTTTGTGCGACAAACAAAA	TTTAGGAGATGACATGCCGC	20	20	30.0	50.0	53.6	58.0	12.1	0.0	4.432278	113165	113676	632-638	1.000000	49.1
rpl32-trnL_p2	rpl32-trnL	2	TTTTTGTGCGACAAACAAAA	TCCTAAGAGCAGCGTGTCTA	20	20	30.0	50.0	53.6	57.9	12.1	0.0	4.510114	113165	113676	577-583	1.000000	48.9
rpl32-trnL_p3	rpl32-trnL	3	TTTTTGTGCGACAAACAAAA	AGCAGCGTGTCTACCAATTT	20	20	30.0	45.0	53.6	57.8	12.1	0.0	4.570841	113165	113676	570-576	1.000000	48.5
rpl32-trnL_p4	rpl32-trnL	4	TTTTTGTGCGACAAACAAAA	CCTAAGAGCAGCGTGTCTAC	20	20	30.0	55.0	53.6	57.8	12.1	0.0	4.582132	113165	113676	576-582	1.000000	48.3
rpl32-trnL_p5	rpl32-trnL	5	TTTTTGTGCGACAAACAAAA	CTAAGAGCAGCGTGTCTACC	20	20	30.0	55.0	53.6	57.8	12.1	0.0	4.582132	113165	113676	575-581	1.000000	48.3
ccsA-ndhD_p1	ccsA-ndhD	1	AATTGTGGCGTCTGTTGGAT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	58.1	57.4	0.0	0.0	0.644670	114825	115056	412-419	1.000000	78.7
ccsA-ndhD_p2	ccsA-ndhD	2	ATTGTGGCGTCTGTTGGATT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	58.1	57.4	0.0	0.0	0.644670	114825	115056	411-418	1.000000	78.7
ccsA-ndhD_p3	ccsA-ndhD	3	TGGCGTCTGTTGGATTTCTT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	57.7	57.4	0.0	0.0	0.824722	114825	115056	407-414	1.000000	77.3
ccsA-ndhD_p4	ccsA-ndhD	4	TTGTGGCGTCTGTTGGATTT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	58.3	57.4	0.0	0.0	0.868016	114825	115056	410-417	1.000000	76.9
ccsA-ndhD_p5	ccsA-ndhD	5	AATTCAGCAATTGTGGCGTC	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	57.6	57.4	7.4	0.0	0.906873	114825	115056	420-427	1.000000	76.5
ycf1_p1	ycf1	1	GTCCAACTCCGAGATGCAAT	ACGAGCTCAAAGGCGTAAAA	20	20	50.0	45.0	58.0	58.1	1.3	15.4	0.156140	126152	126271	990-1005	1.000000	88.2
ycf1_p2	ycf1	2	GTCCAACTCCGAGATGCAAT	CCGTTCAAGAAAAGGCAAGC	20	20	50.0	50.0	58.0	58.2	1.3	0.0	0.258882	126152	126271	1128-1143	1.000000	86.9
ycf1_p3	ycf1	3	TCAGGGGGTTGTGATAGGAG	ACGAGCTCAAAGGCGTAAAA	20	20	55.0	45.0	58.1	58.1	0.0	15.4	0.246727	126152	126271	459-462	1.000000	86.2
ycf1_p4	ycf1	4	GTGCCAAGGTTTCAGACAGA	ACGAGCTCAAAGGCGTAAAA	20	20	50.0	45.0	58.0	58.1	0.0	15.4	0.158984	126152	126271	613-616	1.000000	85.8
ycf1_p5	ycf1	5	GTCCAACTCCGAGATGCAAT	CGTTCAAGAAAAGGCAAGCC	20	20	50.0	50.0	58.0	58.2	1.3	2.0	0.258882	126152	126271	1142-1142	0.250000	47.9
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GTTGAACCCTGTGAATTGCG	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.7	0.084913	150633	150720	731-752	1.000000	91.3
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TAGGAAGAAGCCCCCTCAAA	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.7	0.088183	150633	150720	853-874	1.000000	91.3
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.9	58.0	0.0	7.6	0.059678	150633	150720	329-342	1.000000	86.2
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	TAGGAAGAAGCCCCCTCAAA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.9	58.0	0.0	7.6	0.062948	150633	150720	451-464	1.000000	84.8
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	GGTATAGGAAGAAGCCCCCT	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	57.9	58.0	0.0	7.6	0.129140	150633	150720	455-468	1.000000	84.2
trnI-CAU-rpl23_p1	trnI-CAU-rpl23	1	GAATGGTTAAAGCGCCCAAC	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	58.0	58.0	2.3	0.7	0.043637	150795	150984	417-430	1.000000	91.7
trnI-CAU-rpl23_p2	trnI-CAU-rpl23	2	GTTGAACCCTGTGAATTGCG	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.7	0.084913	150795	150984	731-752	1.000000	91.3
trnI-CAU-rpl23_p3	trnI-CAU-rpl23	3	TAGGAAGAAGCCCCCTCAAA	TTCTACCCTTTCCCGGAAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.7	0.088183	150795	150984	853-874	1.000000	91.3
trnI-CAU-rpl23_p4	trnI-CAU-rpl23	4	GAATGGTTAAAGCGCCCAAC	ACTGTCTACGGTTCCATTGC	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.103963	150795	150984	596-609	1.000000	91.1
trnI-CAU-rpl23_p5	trnI-CAU-rpl23	5	GAATGGTTAAAGCGCCCAAC	GAAGAGTTCGACCCAATGCT	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.109805	150795	150984	362-375	1.000000	91.1