Hotspot

accD-psaI

LSC · 66077-66712 · 636 bp

Back to Prosopis

Pi 0.0583

MarkerSeek score 79.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 33
pi: 0.0583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTCTTAGTGAGT	AGAGGGTAAGTTGCTGAGAGT	45.0	47.6	56.8	58.1	685-724 bp	1.0	69.1
accD-psaI_p2	AAGGGCGTTCTTAGTGAGTT	AGAGGGTAAGTTGCTGAGAGT	45.0	47.6	56.8	58.1	684-723 bp	1.0	69.1
accD-psaI_p3	AAAGGGCGTTCTTAGTGAGT	GGGTAAGTTGCTGAGAGTTGT	45.0	47.6	56.8	57.9	682-721 bp	1.0	69.0
accD-psaI_p4	AAGGGCGTTCTTAGTGAGTT	GGGTAAGTTGCTGAGAGTTGT	45.0	47.6	56.8	57.9	681-720 bp	1.0	69.0
accD-psaI_p5	AAAGGGCGTTCTTAGTGAGTT	AGAGGGTAAGTTGCTGAGAGT	42.9	47.6	57.5	58.1	685-724 bp	1.0	67.0