Hotspot

trnS-GGA-rps4

LSC · 50925-51216 · 292 bp

Back to Prosopis

Pi 0.0563

MarkerSeek score 80.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 16
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	TGACTCTTCACCCATTCCAA	55.0	45.0	58.4	56.4	501-502 bp	1.0	68.0
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	TTGACTCTTCACCCATTCCA	55.0	45.0	58.4	56.4	502-503 bp	1.0	68.0
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	TTGACTCTTCACCCATTCCAA	55.0	42.9	58.4	57.1	502-503 bp	1.0	66.2
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	TTTGACTCTTCACCCATTCCA	55.0	42.9	58.4	57.1	503-504 bp	1.0	66.1
trnS-GGA-rps4_p5	TCACTCGGTTTCTGGGACAT	TGACTCTTCACCCATTCCAA	50.0	45.0	58.7	56.4	528-529 bp	1.0	65.6