Hotspot

psaA-ycf3

LSC · 47782-48622 · 841 bp

Back to Prosopis

Pi 0.047

MarkerSeek score 80.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 39
pi: 0.047
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	987-994 bp	1.0	83.2
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	993-1000 bp	1.0	83.0
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	1005-1012 bp	1.0	82.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1018-1025 bp	1.0	82.8
psaA-ycf3_p5	AGAAATGACCGGGTTTAGCC	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	1011-1018 bp	1.0	82.7