Hotspot

psbZ-trnG-UCC

LSC · 41730-42355 · 626 bp

Back to Prosopis

Pi 0.0615

MarkerSeek score 80.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 37
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GGTATTCTTAATTCTCTCATCTCTTGA	GCGTCTTCTCTTTGGCAAAG	33.3	50.0	56.7	57.7	701-703 bp	1.0	47.0
psbZ-trnG-UCC_p2	TTCTTAATTCTCTCATCTCTTGA	GCGTCTTCTCTTTGGCAAAG	30.4	50.0	52.6	57.7	697-699 bp	1.0	47.0
psbZ-trnG-UCC_p3	GGTATTCTTAATTCTCTCATCTCTTG	GCGTCTTCTCTTTGGCAAAG	34.6	50.0	55.5	57.7	701-703 bp	1.0	47.0
psbZ-trnG-UCC_p4	AGGTATTCTTAATTCTCTCATCTCT	GCGTCTTCTCTTTGGCAAAG	32.0	50.0	54.8	57.7	702-704 bp	1.0	46.9
psbZ-trnG-UCC_p5	AGGTATTCTTAATTCTCTCATCTCTTG	GCGTCTTCTCTTTGGCAAAG	33.3	50.0	56.7	57.7	702-704 bp	1.0	46.9