Hotspot

rpoB-trnC-GCA

LSC · 30396-31639 · 1244 bp

Back to Prosopis

Pi 0.0562

MarkerSeek score 75.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 68
pi: 0.0562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GATACCGGGGCTTTGCAATA	GCCCCTTCCCCAACTAAAAA	50.0	50.0	58.0	58.0	2052-2055 bp	1.0	85.1
rpoB-trnC-GCA_p2	GATACCGGGGCTTTGCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1701-1704 bp	1.0	85.1
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	GCCCCTTCCCCAACTAAAAA	50.0	50.0	58.0	58.0	2168-2171 bp	1.0	84.9
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1817-1820 bp	1.0	84.9
rpoB-trnC-GCA_p5	CCGGGGCTTTGCAATATTTG	GCCCCTTCCCCAACTAAAAA	50.0	50.0	58.1	58.0	2048-2051 bp	1.0	84.8