Hotspot

atpH-atpI

LSC · 16579-18035 · 1457 bp

Back to Prosopis

Pi 0.0447

MarkerSeek score 76.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1457
variable_sites: 63
pi: 0.0447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCGGCAGAAATCAGTGGATT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.9	57.9	1524-1535 bp	1.0	77.6
atpH-atpI_p2	CGGCAGAAATCAGTGGATTC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	56.9	57.9	1523-1534 bp	1.0	75.8
atpH-atpI_p3	CCCAGCAGCAATAACCGAA	TTTTGCAACTTTAGCTGCGG	52.6	45.0	58.1	57.9	1543-1554 bp	1.0	75.7
atpH-atpI_p4	GCGGCAGAAATCAGTGGAT	TTTTGCAACTTTAGCTGCGG	52.6	45.0	58.2	57.9	1524-1535 bp	1.0	75.1
atpH-atpI_p5	CCCAGCAGCAATAACCGAAG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	59.3	57.9	1543-1554 bp	1.0	74.6